Unstained Precision Plus Protein Standards Instruction Manual Precision Plus Protein Standard Plugs, Unstained, Instruction Manual Strep-Tag Technology for Molecular Weight (MW) Determination on Blots Using. Not intended for human or animal diagnostic or therapeutic uses. Protein Ladders and Standards Precast Protein Gels Protein Electrophoresis and Blotting Products Supporting Documents and Resources. The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample. The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. NativeMark Unstained Protein Standard is a ready-to-use protein standard of eight protein bands in the range of 201,200 kDa, designed for molecular weight estimation of proteins using native gel electrophoresis. Under suggested conditions, Unveil Unstained Protein Ladder resolves 12 major bands in 4-12%Bis- Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane. 2.5 μl per well for general Western transferring.Ģ0 mM Tris-phosphate(pH 7.5 at 25☌), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol. Two orange reference bands at 70 kDa and 25 kDa and one green reference band at 10 kDa highlight the blue-stained protein ladder. The ladder is supplied in the gel loading buffer and is ready to use.ģ μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. The PageRuler Plus Prestained Protein Ladder is a mixture of nine (9) blue-, orange- and green-stained proteins (10 to 250 kDa) for use in protein electrophoresis (SDS-PAGE) and western blotting. The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. (Unstained protein ladders have only the bromophenol blue at the dye front to help monitor progress it does not help to assess blotting efficiency.The Unveil Unstained Protein Ladder is a mixture of 12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa. They offer easier identification of protein molecular weights and the ability to monitor migration during electrophoresis. These prestained recombinant protein ladders include three high-intensity reference bands: 25, 50, and 75 kD. When this ladder is analyzed by SDS-PAGE and stained with Coomassie. Calculate the approximate size of your protein by comparing the image of the membrane with the ladder to the image produced by antibody staining Low price but you get a very good quality of protein ladder Applications: Western Blot,Protein purification. CLEARLY Protein Ladder (Unstained) is a mixture of 11 recombinant highly purified proteins.Verify transfer efficiency between the gel and PVDF, nylon, or nitrocellulose membranes.Monitor protein separation during SDS-polyacrylamide gel electrophoresis.In western blotting, prestained protein ladders can be used to: These standards offer absolute molecular weight accuracy confirmed by mass. Precision Plus Protein Unstained Standards contain the unique Strep -tag affinity peptide, which allows detection and molecular weight determination on western blots. Each protein can be stained with a different color for easy identification or labeled with affinity tags for western blot detection. Use Precision Plus Protein WesternC Standards as a combination standard for western blotting and fluorescence detection in SDS-PAGE. These unstained protein standards include three high-intensity reference bands (25, 50, and 75 kD). Recombinant protein ladders are engineered to produce tight bands, evenly spaced molecular weights, and other specific traits. PI62299), which is gentle and designed for quickly removing primary and near-infrared (IR) dyelabeled secondary antibodies from western blots. Fluorescent Western Blot Stripping Buffer (Cat. Although some laboratories continue to use natural protein ladders, recombinant standards are more commonly used. Want to detect PageRuler unstained protein ladders in your chemiluminescence blot. These broad bands are less distinct, which makes them less useful for molecular weight estimation. Prestained natural proteins may produce broader bands than recombinant proteins, especially when they are stained with more than one color. Prestained naturally occurring proteins will vary due to the amount and location of dye binding sites.
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